| Altrincham v Grays Athletic 27/02/2010 | View match | |
| Kettering Town v Grays Athletic 06/03/2010 | View match | |
| Grays Athletic v Hayes & Yeading United 13/03/2010 | View match | |
| Eastbourne Borough v Grays Athletic 20/03/2010 | View match | |
| Grays Athletic v Stevenage Borough FC 23/03/2010 | View match | |
| Cambridge United FC v Grays Athletic 27/03/2010 | View match | |
| Grays Athletic v York City FC 30/03/2010 | View match | |
| Grays Athletic v Kettering Town 03/04/2010 | View match | |
| Luton Town FC v Grays Athletic 05/04/2010 | £5.00 | |
| Grays Athletic v Wrexham FC 10/04/2010 | View match | |
| AFC Wimbledon FC v Grays Athletic 13/04/2010 | View match | |
| York City FC v Grays Athletic 17/04/2010 | View match |