| Barnsley FC v Rotherham United FC 05/09/2000 | View match | |
| Fulham FC v Barnsley FC 10/09/2000 | £4.00 | |
| Crystal Palace FC v Barnsley FC 12/09/2000 | View match | |
| Barnsley FC v Queens Park Rangers FC 16/09/2000 | View match | |
| Barnsley FC v Crewe Alexandra FC 19/09/2000 | View match | |
| Crewe Alexandra FC v Barnsley FC 23/09/2000 | View match | |
| Crewe Alexandra FC v Barnsley FC 26/09/2000 | View match | |
| Barnsley FC v Grimsby Town FC 30/09/2000 | View match | |
| Huddersfield Town FC v Barnsley FC 06/10/2000 | View match | |
| Barnsley FC v Nottingham Forest FC 14/10/2000 | View match | |
| Barnsley FC v Tranmere Rovers FC 17/10/2000 | View match | |
| Gillingham FC v Barnsley FC 21/10/2000 | View match |