| Rushden & Diamonds v Barrow AFC 23/01/2010 | View match | |
| Barrow AFC v Luton Town FC 06/02/2010 | View match | |
| Barrow AFC v Kettering Town 13/02/2010 | View match | |
| Gateshead v Barrow AFC 16/02/2010 | View match | |
| Ebbsfleet United v Barrow AFC 27/02/2010 | View match | |
| Barrow AFC v Cambridge United FC 06/03/2010 | View match | |
| Stevenage Borough FC v Barrow AFC 09/03/2010 | View match | |
| Barrow AFC v Wrexham FC 16/03/2010 | View match | |
| AFC Wimbledon FC v Barrow AFC 23/03/2010 | View match | |
| Barrow AFC v Eastbourne Borough 27/03/2010 | View match | |
| Barrow AFC v Histon 29/03/2010 | View match | |
| Forest Green Rovers v Barrow AFC 31/03/2010 | View match |