| Gloucester City v Rushall Olympic 28/08/2023 | View match | |
| Scarborough Athletic v Gloucester City 02/09/2023 | View match | |
| Gloucester City v Buxton 05/09/2023 | View match | |
| Gloucester City v Farsley Celtic 09/09/2023 | View match | |
| Darlington FC v Gloucester City 23/09/2023 | View match | |
| Gloucester City v Chorley 07/10/2023 | View match | |
| Southport v Gloucester City 14/10/2023 | View match | |
| Gloucester City v Alfreton Town 21/10/2023 | View match | |
| Tamworth v Gloucester City 24/10/2023 | View match | |
| Boston United v Gloucester City 28/10/2023 | View match | |
| Gloucester City v Curzon Ashton 04/11/2023 | View match | |
| Gloucester City v Warrington Town 07/11/2023 | View match |