| Guiseley AFC v Gateshead 25/10/2016 | View match | |
| Aldershot Town F.C v Guiseley AFC 29/10/2016 | View match | |
| Guiseley AFC v Torquay United FC 12/11/2016 | View match | |
| Dover Athletic FC v Guiseley AFC 19/11/2016 | View match | |
| Barrow AFC v Guiseley AFC 22/11/2016 | View match | |
| Guiseley AFC v Boreham Wood 26/11/2016 | View match | |
| York City FC v Guiseley AFC 29/11/2016 | View match | |
| Guiseley AFC v Chester FC 03/12/2016 | View match | |
| Woking FC v Guiseley AFC 17/12/2016 | View match | |
| Guiseley AFC v Lincoln City FC 26/12/2016 | View match | |
| Lincoln City FC v Guiseley AFC 01/01/2017 | View match | |
| Guiseley AFC v Maidstone United (1992) 07/01/2017 | View match |