| Macclesfield Town FC v Guiseley AFC 21/01/2017 | View match | |
| Wrexham FC v Guiseley AFC 04/02/2017 | View match | |
| Guiseley AFC v Eastleigh 11/02/2017 | View match | |
| Guiseley AFC v Sutton United 14/02/2017 | View match | |
| Dagenham & Redbridge v Guiseley AFC 18/02/2017 | View match | |
| Guiseley AFC v North Ferriby United 25/02/2017 | View match | |
| Guiseley AFC v Barrow AFC 28/02/2017 | View match | |
| Torquay United FC v Guiseley AFC 04/03/2017 | View match | |
| Guiseley AFC v Aldershot Town F.C 11/03/2017 | View match | |
| Boreham Wood v Guiseley AFC 18/03/2017 | View match | |
| Gateshead v Guiseley AFC 21/03/2017 | View match | |
| Guiseley AFC v Dover Athletic FC 25/03/2017 | View match |