| Doncaster Rovers FC v Millwall FC 27/08/1959 | View match | |
| Rochdale FC v Millwall FC 29/08/1959 | View match | |
| Millwall FC v Doncaster Rovers FC 31/08/1959 | View match | |
| Millwall FC v Gateshead 05/09/1959 | View match | |
| Bradford Park Avenue v Millwall FC 07/09/1959 | View match | |
| Darlington FC v Millwall FC 12/09/1959 | View match | |
| Millwall FC v Bradford Park Avenue 14/09/1959 | View match | |
| Millwall FC v Aldershot F.C 19/09/1959 | View match | |
| Northampton Town FC v Millwall FC 21/09/1959 | View match | |
| Barrow v Millwall FC 26/09/1959 | View match | |
| Millwall FC v Northampton Town FC 28/09/1959 | View match | |
| Millwall FC v Gillingham FC 03/10/1959 | View match |