| Port Vale FC v Burton Albion FC 19/01/2010 | View match | |
| Port Vale FC v Macclesfield Town FC 23/01/2010 | View match | |
| Rochdale FC v Port Vale FC 26/01/2010 | View match | |
| Port Vale FC v Hereford United 30/01/2010 | View match | |
| Burton Albion FC v Port Vale FC 06/02/2010 | View match | |
| Port Vale FC v Chesterfield FC 09/02/2010 | View match | |
| Torquay United FC v Port Vale FC 13/02/2010 | View match | |
| Port Vale FC v Barnet FC 20/02/2010 | View match | |
| Port Vale FC v Lincoln City FC 23/02/2010 | View match | |
| Morecambe FC v Port Vale FC 27/02/2010 | View match | |
| Darlington FC v Port Vale FC 02/03/2010 | View match | |
| Port Vale FC v Northampton Town FC 06/03/2010 | View match |