| Darlington FC v Cardiff City FC 29/01/1927 | View match | |
| Derby County FC v Millwall FC 29/01/1927 | View match | |
| Fulham FC v Burnley FC 29/01/1927 | View match | |
| Hull City FC v Everton FC 29/01/1927 | View match | |
| Leeds United FC v Bolton Wanderers FC 29/01/1927 | View match | |
| Liverpool FC v Southport 29/01/1927 | View match | |
| Port Vale FC v Arsenal FC 29/01/1927 | View match | |
| Preston North End FC v Middlesbrough FC 29/01/1927 | View match | |
| Reading FC v Portsmouth FC 29/01/1927 | View match | |
| The Wednesday v South Shields AFC 29/01/1927 | View match | |
| Southampton FC v Birmingham 29/01/1927 | View match | |
| West Ham United FC v Brentford FC 29/01/1927 | View match |