| Clyde v Partick Thistle FC 19/02/1927 | View match | |
| Alloa Athletic FC v Arthurlie 19/02/1927 | View match | |
| St Johnstone FC v Morton FC 19/02/1927 | View match | |
| The Wednesday v Sunderland FC 19/02/1927 | View match | |
| Wigan Borough v Southport 19/02/1927 | View match | |
| Rochdale FC v Stockport County FC 19/02/1927 | View match | |
| Barnsley FC v Clapton Orient 19/02/1927 | View match | |
| Walsall FC v Nelson 19/02/1927 | View match | |
| Doncaster Rovers FC v Wrexham FC 19/02/1927 | View match | |
| Accrington Stanley (1891) v Barrow 19/02/1927 | View match | |
| Durham City v Crewe Alexandra FC 19/02/1927 | View match | |
| Raith Rovers FC v Clydebank FC (Old) 19/02/1927 | View match |