| Queens Park Rangers FC v Southampton FC 01/08/2009 | View match | |
| Southampton FC v Millwall FC 08/08/2009 | View match | |
| Southampton FC v Northampton Town FC 11/08/2009 | View match | |
| Huddersfield Town FC v Southampton FC 15/08/2009 | View match | |
| Swindon Town FC v Southampton FC 18/08/2009 | View match | |
| Southampton FC v Brentford FC 22/08/2009 | View match | |
| Southampton FC v Birmingham City FC 25/08/2009 | £4.80 | |
| Southampton FC v Brentford FC 28/08/2009 | View match | |
| Stockport County FC v Southampton FC 29/08/2009 | View match | |
| Southampton FC v Colchester United FC 05/09/2009 | £4.80 | |
| Charlton Athletic FC v Southampton FC 12/09/2009 | £6.50 | |
| Southampton FC v Yeovil Town FC 19/09/2009 | £4.80 |