| Southport v Leamington 11/01/2025 | View match | |
| Hereford F.C. v Southport 18/01/2025 | View match | |
| Southport v Chorley 25/01/2025 | View match | |
| Southport v Spennymoor Town F.C. 28/01/2025 | View match | |
| Buxton v Southport 01/02/2025 | View match | |
| Radcliffe Borough v Southport 08/02/2025 | View match | |
| Southport v Darlington FC 15/02/2025 | View match | |
| Brackley Town v Southport 22/02/2025 | View match | |
| Southport v Curzon Ashton 25/02/2025 | View match | |
| Peterborough Sports v Southport 01/03/2025 | View match | |
| Southport v Kings Lynn Town 08/03/2025 | View match | |
| Marine v Southport 11/03/2025 | View match |