| Southampton FC v Brentford FC 28/08/2009 | View match | |
| Stockport County FC v Southampton FC 29/08/2009 | View match | |
| Southampton FC v Colchester United FC 05/09/2009 | £4.80 | |
| Charlton Athletic FC v Southampton FC 12/09/2009 | £6.50 | |
| Southampton FC v Yeovil Town FC 19/09/2009 | £4.80 | |
| Carlisle United FC v Southampton FC 26/09/2009 | View match | |
| Southampton FC v Bristol Rovers FC 29/09/2009 | View match | |
| Southampton FC v Gillingham FC 03/10/2009 | View match | |
| Southend United FC v Southampton FC 09/10/2009 | View match | |
| Oldham Athletic FC v Southampton FC 17/10/2009 | View match | |
| Southampton FC v MK Dons (Milton Keynes Dons) 24/10/2009 | £4.90 | |
| Leyton Orient FC v Southampton FC 31/10/2009 | View match |