| Southport v Yeovil Town FC 26/08/2000 | View match | |
| Rushden & Diamonds v Southport 28/08/2000 | View match | |
| Chester City FC v Southport 02/09/2000 | View match | |
| Southport v Scarborough FC 05/09/2000 | View match | |
| Southport v AFC Telford Utd 09/09/2000 | View match | |
| Doncaster Rovers FC v Southport 12/09/2000 | View match | |
| Southport v Woking FC 23/09/2000 | View match | |
| Southport v Boston United 26/09/2000 | View match | |
| Nuneaton Borough v Southport 30/09/2000 | View match | |
| Northwich Victoria v Southport 03/10/2000 | View match | |
| Kingstonian FC v Southport 14/10/2000 | View match | |
| Kettering Town v Southport 21/10/2000 | View match |