| Southport v Dover Athletic FC 11/11/2000 | View match | |
| Havant & Waterlooville v Southport 18/11/2000 | View match | |
| Dagenham & Redbridge v Southport 25/11/2000 | View match | |
| Southport v Doncaster Rovers FC 28/11/2000 | View match | |
| Southport v Kingstonian FC 09/12/2000 | View match | |
| Leigh RMI v Southport 16/12/2000 | View match | |
| Stevenage Borough FC v Southport 22/12/2000 | View match | |
| Southport v Morecambe FC 26/12/2000 | View match | |
| Morecambe FC v Southport 01/01/2001 | View match | |
| Southport v Rushden & Diamonds 06/01/2001 | View match | |
| Yeovil Town FC v Southport 20/01/2001 | View match | |
| Scarborough FC v Southport 27/01/2001 | View match |