| Maidstone United (1992) v Southport 04/03/2017 | View match | |
| Southport v Torquay United FC 11/03/2017 | View match | |
| Braintree Town v Southport 18/03/2017 | View match | |
| York City FC v Southport 21/03/2017 | View match | |
| Southport v Boreham Wood 25/03/2017 | View match | |
| Southport v Guiseley AFC 01/04/2017 | View match | |
| Southport v Macclesfield Town FC 04/04/2017 | View match | |
| Sutton United v Southport 08/04/2017 | View match | |
| Dover Athletic FC v Southport 14/04/2017 | View match | |
| Southport v Forest Green Rovers 17/04/2017 | View match | |
| Tranmere Rovers FC v Southport 22/04/2017 | View match | |
| Southport v Lincoln City FC 29/04/2017 | View match |